Oxidation of methionine residues of recombinant human interleukin 2 in aqueous solutions.
نویسندگان
چکیده
منابع مشابه
Oxidation of methionine residues in proteins of activated human neutrophils.
A simple assay for the detection of 35S-labeled methionine sulfoxide residues in proteins is described. The assay, which is based on the ability of CNBr to react with methionine but not with methionine sulfoxide, requires the prelabeling of cellular proteins with [35S]methionine. The assay was used to study the extent of methionine oxidation in newly synthesized proteins of both activated and q...
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Backgrounds and objectives: Interleukin 2 (IL-2) secreted by activated CD4+ T cells has been known as a major mediator in both adaptive and native immune system due to a board range of effects on different cells in the immunity system (1-6). Methods: cDNA synthesis was performed using gene- specific primers designed by Gene Runner software after RNA extraction of mouse splenocytes. PCR pro...
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In order to increase the productivity of human interleukin-2 (IL-2) as a model protein in the recombinant E.coliBL21 (DE3), the effect of magnesium acetate and manganese acetate was studied based on full factorial design experiments magnesium acetate and manganese acetate and their interaction exhibited an increasing effect on IL-2 expression level upto 25.24%, 16.80% and 23.30%, respective...
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Introduction: Since the use of antibiotics and the release of their residuals to the environment have extensively been growing, it is expected to see mutagenated and resistant bacteria, which are a serious threat to the human health and natural ecosystems. In this study, a novel electrochemical advanced oxidation process by means of the β-PbO2 was employed in order to decompose ciprofloxacin. M...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1989
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.37.2160